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Bio-Technology Engineering - Faculty Publication

Name of the Staff: A. Haseena , V.M. Nishad , M. Balasundaran

Name of the journal: IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT)

Tittle of the journal:

A consortium of Thermophilic microorganisms for aerobic composting

Year of Publication: 2016

Abstract:

Accumulation of waste materials is a major crisis in the present world causing increased environmental threat. Aerobic composting is the process of conversion of solid waste materials into stable compost by the activity of aerobic microorganisms. Thermophilic microorganisms involved in aerobic composting, especially which encompass elevated ability for the production of waste degrading enzymes play a superior role in escalating the degradation rate. A consortium of 14 different thermophilic microorganisms which is having the maximum enzyme production was selected for composting weeds, herbal medicinal waste materials, coir pith and sawdust. The consortium could increase the rate of degradation by 30 to 40 % for weed waste, herbal medicinal waste and coirpith. In the case of sawdust also the selected consortium could reduce the C/N ratio, but could not make final quality compost.

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Name of the Staff: Francis. N. K

Name of the journal: International journal of Materials and Manufacturing Process

Tittle of the journal:

Swirling abrasive fluidized bed machining: Effect of process parameters on machining performance

Year of Publication: 2015

Abstract:

                Swirling Abrasive Fluidized Bed Machining (SA-FBM) is a novel variant of Fluidized Bed Machining (FBM). This research focuses on the experiments performed on copper specimens using silicon carbide abrasive particles to investigate the influence of operating parameters such as grain size, superficial velocity, and machining time on metal removal rate, transformation of surface texture, and the surface finish. The study concludes that the surface modification is faster with SA-FBM compared with conventional FBM; the initial roughness conditions of the workpiece have no effect on the maximum possible surface finish; moreover, for faster metal removal, higher superficial velocity, and for better surface finish, fine abrasive grains are preferred.

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Name of the Staff: Francis. N. K

Name of the journal: International journal of Materials and Manufacturing Process

Tittle of the journal:

SAFBM of Softer Materials: An Investigation into Micro-Cutting Mechanisms and the Evolution of Surface Roughness Profile

Year of Publication: 2015

Abstract:

                Swirling Fluidized Bed Machining (SAFBM) is a nontraditional machining process and novel variant of Fluidized Bed Machining (FBM) in which the former has overcome certain drawbacks of the latter such as roughness variation and shading effect. SAFBM generates significant material removal and surface finish on the workpiece surface during the machining process with consistency and flexibility. 10 The present detailed study emphasizes on the machining of softer materials such as brass and aluminum using abrasive particles such as silicon carbide in order to analyze the effect of various micro-cutting mechanisms on the generation of surface texture. This study examines the evolution of surface roughness profile after progressive machining with abrasives ranging from coarse, medium and fine grades of SiC with the help of optical microscopic images of the machined surface. The research concludes that using SAFBM, flat and uniform surface finishing with modification ratio in terms of roughness parameters ranging from 5 to 7 is possible within 7–8 hours of processing.

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Name of the Staff: Francis. N. K

Name of the journal: IJAER, Research India Publications.

Tittle of the journal:

Optimizing Abrasive Particle Speed and Size on Machining Performance in SAFBM of Brass

Year of Publication: 2015

Abstract:

                Swirling Abrasive Fluidized Bed Machining (SAFBM) is a non-traditional method of abrasive flow machining as well as a novel variant of Fluidized Bed Machining (FBM) which is used to machine complex shape and size of work piece that are difficult to machine with conventional method. Owing to its ability to perform machining and generate polished surface from a roughness value of Ra 1.2µ to 0.2 µ within 8 hours of processing, this new method offers greater scope in the surface modification of rough machined surfaces with complex geometry such as component with ducts and grooves. The effects of various process parameters like machining time, abrasive grain size and particle impact speed have been investigated to reveal their impact on metal removal rate using Taguchi methodology on brass specimen. The experimental layout was designed based on the Taguchi?s L9 (34 ) Orthogonal array technique and analysis of variance (ANOVA) was performed to identify the effect of the cutting parameters on the response variables. The optimum set of process parameters has also been predicted to maximize the MRR

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Name of the Staff: Francis. N. K

Name of the journal: Journal IJARTET

Tittle of the journal:

Investigating the effect of material properties on machining performance in swirling fluidized bed machining

Year of Publication: 2015

Abstract:

Swirling Fluidized Bed Machining (SFBM) is a novel variant of Fluidized Bed Machining (FBM) in which the former has overcome certain drawbacks of the latter, such as machining variation across the due to the preferential abrasive action at the bottom portion just above the porous distributer and the shading effect due to the work piece geometry. This research focuses on the experiments performed on the specimens of soft and aluminum using silicon carbide abrasive particles with the aim of investigating the influence of material hardness on surface finish and metal removal. Further investigation on the feasibility of industrial application as well as analysis was also carried out.

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Name of the Staff: Francis. N. K

Name of the journal: Journal, Applied Mechanics and Materials

Tittle of the journal: Swirling Fluidized Bed Polishing: A New Non-Conventional Method of Surface Modification

Year of Publication: 2015

Abstract:

Swirling Fluidized Bed Polishing (SFBP) is a non–traditional alternative abrasive flow surface finishing form of Fluidized Bed Machining (FBM) in which the former has special features to overcome certain significant limitations of the latter, namely the variation of the surface roughness vertically along the component surface and the screening effect owing to the complex contours across the work piece geometry. Owing to its ability to perform machining and generate finely polished surface from a roughness value of Ra 1.2µ to 0.2 µ within 8 hours of processing, this new method offers greater scope for surface modification of rough machined surfaces with ducts and grooves. This research focus on investigating the effect of abrasive particle concentration on metal removal rate per unit area of the specimen surface. 3D surface morphology analysis investigates the quality of the polished surface and the study of circumferential uniformity and the analysis of machining accuracy of a complex-contoured component further examine its scope and relevance in industrial applications.

Name of the Staff: H. Harikrishnan

Name of the journal: Journal of Basic Microbiology

Title of the journal:, Enhanced Production of phenazine-like metabolite produced by Streptomyces aurantiogriseus VSMGT1014 against rice pathogen, Rhizoctonia solani.

Year of Publication: 2015

Abstract: The efficacy of a rhizobacterium Streptomyces aurantiogriseusVSMGT1014 for the production of bioactive metabolites with antifungal properties was evaluated under in vitro conditions. The production of bioactive metabolites by S. aurantiogriseus VSMGT1014 in International Streptomyces Project-2 (ISP-2) broth, supplemented with glucose and ammonium acetate was found to be the most suitable carbon and nitrogen sources for the maximum production of bioactive metabolites against rice pathogen, Rhizoctonia solani. The zone of inhibition range from 23.5 to 28.5mm and 10.3 to 18.3mm for glucose and ammonium acetate supplemented media, respectively. The culture filtrate of S. aurantiogriseusVSMGT1014 at pH 7.5, 37 °C at 120 rpm in 6 days of incubation showed the maximum production of bioactive metabolites with antagonistic potential. The crude metabolite was characterized by different spectral studies such as Ultraviolet spectrum, infrared-spectrum and based on the different analytical techniques, including thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) with the retention time 29.4 and the bioactive metabolite was identified as phenazine, which was confirmed by pure phenazine compound as positive control.

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Name of the Staff: H. Harikrishnan

Name of the journal: South Indian Journal of Biological Sciences, 2015.

Title of the journal: Improvement of concrete durability by bacterial carbonate precipitation.

Year of Publication: 2015

Abstract:

As like humans having the ability to precipitate minerals in the form of bones and teeth continuously, even some bacteria like Bacillus pasteurii and Bacillus sphaericus can continuously precipitate calcite, called as microbiological induced calcite precipitation. The present investigation discusses on the effects of B. pasteurii and B. sphaericus on durability of concrete beams. The efficacy of the bio deposition treatment on concrete beams with bacteria, alkali, sulfate and freeze-thaw environments along with water absorption was studied. The presence of a layer of calcium carbonate and microbial biomass resulted in a decrease of permeation properties of cementation materials. This technique not only improved the concrete strength but also enhanced water impermeability through calcite precipitation, quantified by X-ray diffraction analysis. Hence this biological treatment is highly desirable and eco-friendly. The present work guarantees a biological metabolic outcome with improvement in concrete and building material technology.

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Name of the Staff: H. Harikrishnan

Name of the journal: Digest Journal of Nanomaterials and Biostructures

Title of the journal: Facile synthesis of silver nanoparticles using Streptomyces sp. VSMGT 1014 and their antimicrobial efficiency.

Year of Publication: 2015

Abstract:

Microbial synthesis of nanoparticles has a prospective to develop simple, cost-effective and eco-friendly method for production of technologically important materials. The present investigation demonstrates the role of rice rhizosphere  Streptomyces  sp. mediated silver nanoparticles in bio control of various  plant  fungal pathogens  and  nosocomial infection causing bacteria  by  in vitro  antagonistic activity  and agar well diffusion method respectively. Isolated actinomycetes were screened for antagonism against plant fungal pathogens and the potent strain was further screened against human pathogenic bacteria. The potential strain, VSMGT1014 was identified by microscopic, physiological, biochemical characteristics and used for synthesis of silver nanoparticles. The nanoparticles were characterised by UV-Vis Absorption spectroscopy, AFM, FTIR and X-ray diffraction. The AgNPs were determined to be spherical through the observation under AFM.  X-ray diffraction patterns displayed typical peaks from 24°-85° at position 2 ? for crystalline silver. It was found that the synthesised nanoparticles showed MIC range of 10-50µg/mL against selected plant fungal pathogens.  Moreover these nanoparticles showed significant level of inhibition as MIC range from 10- 25 µg/mL against clinically important pathogenic bacteria. Through this investigation, we concluding with Streptomyces sp. VSMGT1014 mediated silver nanoparticles would be a potential nanomedicine against various pathogenic organisms.

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Name of the Staff: H. Harikrishnan

Name of the journal: World Journal of Microbiology and Biotechnology

Title of the journal: Antagonistic potential of native strain Streptomyces aurantiogriseus VSMGT1014 against sheath blight of rice disease.

Year of Publication: 2014

Abstract:

A total of 132 actinomycetes was isolated from different rice rhizosphere soils of Tamil Nadu, India, among which 57 showed antagonistic activity towards Rhizoctonia solani, which is sheath blight (ShB) pathogen of rice and other fungal pathogens such as Macrophomina phaseolina, Fusarium oxysporum, Fusarium udum and Alternaria alternata with a variable zone of inhibition. Potential actinomycete strain VSMGT1014 was identified as Streptomyces aurantiogriseusVSMGT1014 based on the morphological, physiological, biochemical and 16S rRNA sequence analysis. The strain VSMGT1014 produced lytic enzymes, secondary metabolites, siderophore, volatile substance and indole acetic acid. Crude metabolites of VSMGT1014 showed activity against R. solani at 5µgml-1; however, the prominent inhibition zone was observed from 40 to 100µgml-1. Reduced lesion heights observed in culture, cells-free filtrate, crude metabolites and carbendazim on challenge with pathogen in the detached leaf assay. The high content screening test clearly indicated denucleation of R. solani at 5µgml-1 treatment of crude metabolite and carbendazim respectively. The results conclude that strain VSMGT1014 was found to be a potential candidate for the control of ShB of rice as a bio fungicide.

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Name of the Staff: H. Harikrishnan

Name of the journal: International Journal of Current Microbiology and Applied Sciences Title of the journal: Optimization for production of Indole acetic acid (IAA) by plant growth promoting Streptomyces sp VSMGT1014 isolated from rice rhizosphere.

Year of Publication: 2014

Abstract:

     The present study focused on the characterization of Indole acetic acid (IAA) production under in vitro condition. A total of 90 actinomycetes was isolated from rice rhizosphere, which was collected from rice fields in the southern districts of Tamil Nadu, India.  All isolates were screened for antagonism towards phytofungal pathogens such as Rhizoctonia solani, Macrophomina phaseolina, Fusarium oxysporum, Fusarium udum and Alternaria alternata.  Out of 95 isolates, 65 were found to be producing IAA was confirmed by colorimetric method.  Isolate VSMGT1014 produced IAA in the ISP-2 medium supplemented with 0.5% L -tryptophan in the amount of 15.96 µg/ml.  The ISP-2 medium was recorded as the best medium for production of IAA, where the maximum IAA production was recorded at 30 °C and pH 8 for the production of 4.76µg/ml and 26.63 µg/ml respectively. The specific spot was found from the extracted IAA has similarity to authentic IAA with the same Rf value of 0.92. High performance liquid chromatography (HPLC) was carried out compared with authentic IAA at 17.5 min retention time was recorded.  The selected strain VSMGT1014 could significantly enhance the growth of rice plants compared with that of non-inoculated plants. The results showed that an increase in root and shoot length 52% and 54.55% respectively compared to control. Overall, the results of this study indicate that potential of VSMGT1014 as a significant plant growth substance producer and good inoculants for the growth of rice seedlings.

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Name of the Staff: H. Harikrishnan

Name of the journal: BMC Infectious diseases

Title of the journal: Larvicidal and antimicrobial activities of silver nanoparticles synthesized using marine fluorescent pseudomonads.

Year of Publication: 2014

Abstract:

Research on nanoparticles opens up newer avenues for unravelling various biological challenges including the control of infectious diseases. The present investigation has been aimed to study larvicidal and antimicrobial activities of silver nanoparticles (AgNP’s) synthesized using marine fluorescent pseudomonads. With the suppression of growth of filarial nematode in the larval stage, AgNP’s has the potential in eradicating this vector borne disease. It is clear that this isolate can be used to synthesize bioactive nanoparticles efficiently in an eco friendly and cost effective manner besides the fact that it can be used for the control of human and plant pathogenic microbes.

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Name of the Staff: H. Harikrishnan

Name of the journal: Journal of Optoelectronics and Biomedical Materials

Title of the journal: In vitro eco friendly synthesis of cadmium sulfide nanoparticles using heterotrophic Bacillus cereus.

Year of Publication: 2014

Abstract:

Biological application of nanoparticles is very much interesting and increasingly   recognized for their utility especially in bio-nanomedicine. The present investigation was biological synthesis of cadmium nanoparticles using Bacillus cereus and toxic effect on prokaryotic pathogens. UV spectrum, XRD and SEM analysis of the cadmium sulfide nanoparticles indicated that they are in varying size from 30 to 100 nm size. Antibacterial activity of CdS nanoparticles against three clinical isolates of Gram positive Staphylococcus aureus bacterial isolates and two Pseudomonas aeruginosa, isolates analyzed. Gram positive Staphylococcus aureus was sensitive than Gram negative Pseudomonas aeruginosa.  Pseudomonas species showed higher inhibitory effect than Staphylococcal isolates.

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Name of the Staff: H. Harikrishnan

Name of the journal: Bioorganic and Medicinal Chemistry Letters

Title of the journal: An eco-friendly and water mediated product selective synthesis of 2-aminopyrimidines and their in-vitro anti-bacterial evaluation.

Year of Publication: 2014

Abstract:

A greener water mediated protocol for the efficient synthesis of a library of 2-amino-6-styryl pyrimidines (4) and their dihydro analogues (3) has been reported. Most of the saturated compounds (3) rather than their unsaturated analogues (4) showed better anti-bacterial (in vitro) activity against three human pathogens viz. Staphylococcus aureus, Klebsiella pneumonia and Escherichia coli. In particular, three of them (3b, 3i&3k) exhibited high inhibition against the growth of all the three pathogens comparable with that of the reference drug, tetracycline.

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Name of the Staff: H. Harikrishnan

Name of the journal: Chinese Chemical Letters

Title of the journal: An  eco-friendly  water  mediated  synthesis  of  1,2,3-triazolyl-2-4  aminopyrimidine  hybrids  as  highly  potent  anti-bacterial  agents.

Year of Publication: 2014

Abstract:

An elegant and efficient synthesis of novel 1, 2, 3-triazole fused 2-aminopyrimidine hybrids has been accomplished for the first time in the green solvent viz. water. The hybrid molecules exhibit significant anti-bacterial activity when screened against three human pathogens viz. Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. In comparison to the commercially marketed drug tetracycline, some of them are equally potent and a few are more potent.

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Name of the Staff: H. Harikrishnan

Name of the journal: Chalcogenide Letters

Title of the journal: Microbial synthesis of Selinium nanocomposite using Saccharomyces cerevisiae and its antimicrobial activity against pathogens causing nosocomial infection.

Year of Publication: 2012

Abstract:

Microbial synthesis of nanocomposite is a green chemistry eco-friendly method. It is a branch of nanoscience for biomedical applications that interconnects nanotechnology and microbial biotechnology. It is only recently that microorganisms have been explored as potential bio factories for synthesis of metal nanoparticles and nanocomposites. By this method, the production efficiency of nanoparticles is very high permitting to relatively easy scale-up the process. Biosynthesis of selenium nanocomposites in aerobic conditions by yeast, Saccharomyces cerevisiae, is reported in the present study. Treating sodium selenite solutions with S. cerevisiae cells and rapid reduction leads to the formation of highly stable selenium nanoparticles in solution. UV spectrum, XRD and SEM analysis of the selenium nanocomposites indicated that they are in the range of size from 30 to 100 nm size. The nanocomposites were further analyzed for antimicrobial activity against a panel of nosocomial infection causing pathogenic bacteria, and they exhibited significant antimicrobial activity.

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Name of the Staff: H. Harikrishnan

Name of the journal: Journal of Bioscience Research

Title of the journal: Utilization of waste ripe Banana, and peels for Bio ethanol production using Saccharomyces cerevisiae.

Year of Publication: 2011

Abstract:

Dried and ground peel biomass, ripe waste banana and Hydrolyzed peels of green and red banana used for bio ethanol production by Saccharomyces cerevisiae with shake flask culture. The substrate was given with different concentration as 1%, 2.5%, 5%, 7.5% and 10% (w/v) along with 1% inoculum. Saccharomyces cerevisiae exhibited maximum yield of ethanol in ripened red banana and their hydrolyzed peels about 1.3% and 0.27% (v/v) in 10% substrate concentration. The minimum yield about 0.02% of alcohol was produced in green un hydrolyzed banana peels with 1% substrate concentration. Conversion of reducing sugar range was 21% (g %) in un hydrolyzed green banana peels and maximum of 76% in red ripened banana as a substrate.

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Name of the Staff: H. Harikrishnan

Name of the book: Sustainable approaches to controlling plant pathogenic bacteria

Title of the chapter: Biocontrol mechanisms of siderophores against bacterial plant pathogens.

Name of the Publisher: CRC Press, Taylor and Francis

Year of Publication: 2015

Abstract:

Siderophore is an iron-healing compound that has an immense role in microbial interaction, especially in the rhizosphere. Siderophore is present in one of the major mechanisms of bacteria that is involved in the biological control of plant diseases. Both plant pathogenic fungi and bacteria are reported to be inhibited by siderophore-producing biocontrol agents. These siderophores are produced in iron-limited conditions to sequester the less-available iron from the environment and thereby deprive the pathogen of iron, which ultimately leads to inhibition. Three main groups of siderophores have been reported: hydroxy-mate siderophore, catecholate siderophore, and mixed siderophore. Siderophores are synthesized through two different biosynthetic pathways: NRPSs-dependent and non-NRPSs-dependent. The ABC-type transporter proteins are also involved in the delivery of iron siderophore   complex into the cytosol of the producing organism, where it can be utilized. Some pathogenic bacteria capable of producing siderophores are highly virulent. Siderophore inter-acts with the H2O2 and peroxidases in the affected tissue either to enhance oxidative stress induced by harpin, coded by an hrp N gene, or to protect bacterial cells by inhibiting the generation of reactive oxygen species. In this chapter we discuss control of bacterial plant pathogens with the production of siderophore by antagonistic rhizobacterium. Emphasis is given to the most economically important bacterial plant diseases such as bacterial soft rot of potato, tomato bacterial wilt, bacterial canker of tomato, rice bacterial blight, bacterial blight of   cotton, bacterial leaf spot of mungbean, and fire blight disease in apple. An overview is included of the significance of siderophores in the context of the inhibition of plant pathogenic bacteria.

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Name of the Staff: H. Harikrishnan

Name of the book: Prospects in Bioscience: Addressing the issues

Title of the chapter: Streptomyces sp.VSMGT1014 mediated antifungal activity against fungal plant pathogens .

Name of the Publisher: Springer

Year of Publication: 2013

Abstract:

Presently actinomycetes from rice rhizosphere are screened for novel bioactive secondary metabolites and for bio control potential against fungal plant pathogens. A total of 102 actinomycetes were isolated from rice rhizosphere soils collected from Tamil Nadu, India. All the isolates were screened against sheath blight pathogen Rhizoctonia solani by dual culture method with inhibition zone range from 0.6cm to 3.0 cm. Among 102, 56 have shown the antagonistic activity against R. solani. Due to superior bio control activity against fungal pathogens over the other actinomycetes, an efficient strain VSMGT1014 was selected and identified as Streptomyces sp based on the biochemical and Atomic force microscope observation. The culture filtrate of Streptomyces sp. VSMGT1014 remarkably inhibited the mycelial growth, conidial and sclerotial germination of many fungal pathogens such as Macrophomina phaseolina, Fusarium oxysporum, Fusarium udum and Alternaria alternata. The strain Streptomyces sp.VSMGT1014 produced five different compounds with Rf value range 0.46, 0.51, 0.61, 0.85 and 0.89. Ethyl acetate extract of crude metabolite from VSMGT1014 significantly inhibited the mycelia growth of most of the fungal pathogens as well as sclerotial germination of R. solani.

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Name of the Staff: H. Harikrishnan

Name of the book: Prospects in Bioscience: Addressing the issues

Title of the chapter: Evaluation of Pseudomonas sp. VSMKU - 4046 for suppression of sheath blight (ShB) of rice in detached leaf assay.

Name of the Publisher: Springer

Year of Publication: 2013

Abstract:

The study of root-associated bacteria and their antagonistic potential is important not only for understanding their ecological role in the rhizospheric and the interaction with plants but also for the suppression of soil borne plant pathogens. In this context a total of 206 Fluorescent pseudomonads (FPs) were isolated from rice rhizosphere in Southern part of Tamil Nadu.  Initially 113 isolates were found which active against Rhizoctonia solani, of which   89 were strongly active against R. solani with Zone of inhibition range from 10mm to 32mm.  Production of lytic enzymes such as Chitinase, Cellulase, Protease, Amylase, Pectinase, Phosphate solubilisation, Hydrogen cyanide, Siderophore and IAA   were recorded with 89 antagonistic fluorescent pseudomonads. Antagonistic fluorescent pseudomonads were also tested towards other fungal pathogens such as Macrophomina phaseolina, Fusarium oxysporum, Fusarium udum and Alternaria alternata. Among 89 the strain VSMKU- 4046 has high antagonistic potential against R. solani. Strain VSMKU- 4046 significantly control sheath blight of rice compare to control using sclerotia of   R.solani through detached leaf assay. The lesion length around the sclerotium was measured and ShB severity was rated by the Relative Lesion Height (RLH) method with the following formula: % RLH = 100 x Total height of lesions / Total leaf height.

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Name of the Staff: H. Harikrishnan

Name of the book: Microbiological Research In Agroecosystem Management

Title of the chapter: Characterization of Groundnut Rhizosphere Pseudomonas sp. VSMKU 2013 for Control of Phytopathogens.

Name of the Publisher: Springer

Year of Publication: 2013

Abstract:

A total of 140 Fluorescent pseudomonads spp. isolated from rhizosphere soil of groundnut. All isolates were evaluated in vitro for selection of potential antagonistic fluorescent pseudomonads (FPs) against fungal pathogens. Among 140 FPs, 82 FPs isolates were showed antagonistic activity against Macrophomina phaseolina for primary screening with zone of inhibition range 0.9cm to 2.9cm. For secondary screening of other fungal pathogens such as Rhizoctonia solani, Fusarium oxysporum, Fusarium udum and Alternaria alternata with zone of inhibition range 0.7cm to 1.9cm, 0.7cm to 2.9cm, 0.9 cmto2.9cm and 0.2cm to 3.0cm. All antagonistic fluorescent pseudomonads were screened for the production of Indole acetic acid (59/82), Hydrogen cyanide (61/82), Siderophore (40/82), Phosphate solubilization (66/82), Hydrolytic enzymes such as   Protease (51/82), Amylase (4/82), Gelatinase (51/82), Cellulase (48/82) and Pectinase (3/82). Among 82 antagonistic bacteria, the strain designates as VSMKU-2013 selected for further studies based on the antagonistic potential. The strain VSMKU-2013 was identified as Pseudomonas sp. based on the biochemical and morphological characters.  The germination, growth promotion of groundnut seeds and identification of IAA genes are in progress.

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Name of the Staff: H. Harikrishnan

Name of the book: Current Scenario in Biotechnology

Title of the chapter: Phenazine Antibiotic producing Pseudomonas sp VSMKU 4047 against Sheath Blight of Rice.

Name of the Publisher: Bloomsbury Publications

Year of Publication: 2012

Abstract:

Sheath blight of rice is the one of the most overwhelming disease caused by Rhizoctonia solani. Heterocyclic nitrogen-containing secondary metabolite phenazine produced by Fluorescent pseudomonades (FPs). Initially 63   Fluorescent pseudomonades were isolated from rice rhizosphere of Dindugal district, Tamil Nadu. Among 63, Phenazine antibiotic genes were identified through Polymerase chain reaction on 15 FPs using   universal primer of phenazine. Out of 63, the strain VSMKU – 4047, was identified as Pseudomonas sp. Based on the biochemical, morphological character and 16s rDNA sequence analysis. Owing to broad antagonistic concert, the strain VSMKU-4047 was selected for further studies. Production of Phosphate solubilization, Hydrogen cyanide and Siderophore was recorded. Lytic enzymes production by the strain VSMKU-4047 was regarded such as Chitinase, Cellulase, Protease, Amylase and Pectinase.. Biosynthesis of Phenazine was visualized   on Thin Layer Chromatography (TLC) with Rf value 0.5 compare with that of authentic Phenazine. TLC product of Phenazine exerted potent in vitro antifungal activity against Rhizoctonia solani.

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Name of the Staff: H. Harikrishnan

Name of the book: Current Scenario in Biotechnology

Title of the chapter: Preliminary Screening of Fluorescent Pseudomonads from Groundnut rhizosphere against fungal pathogens and phenazine gene identification by Polymerase Chain reaction (PCR).

Name of the Publisher: Bloomsbury Publications

Year of Publication: 2012

Abstract:

Fluorescent Pseudomonades (FPs) may comprise of mixed populations of naturally occurring microbes that can be applied as inoculants to increase soil microbial diversity. Microbial community of Pseudomonades in soil ecosystem improves soil quality, soil health, growth, yield and quality of crops. Groundnut rhizosphere microbial populations may consists of ideal species of microorganisms including plant growth promotion, plant disease management, soil toxicant degrading microbes, and other useful microbes. Efficient and potential soil microbial biota is only suitable for sustainable agriculture practices.  The aim of the present study is to explore the antifungal efficiency of FPs against fungal pathogens.  A total of 386 FPs isolated from groundnut rhizosphere of southern district of Tamil Nadu. Out of 386, in preliminary screening, 92 fluorescent pseudomonades were showed antagonistic activity against charcoal rot groundnut pathogen Macrophomina phaseolina. Among 92 based on the superior antagonistic performance, the strain VSMKU-2013 was selected for further studies. The strain VSMKU-2013 was identified as Pseudomonas sp., based on the biochemical, morphological and 16s rDNA sequence analysis. Production of Indole acetic acid, Phosphate solubilization, Hydrogen cyanide and Siderophore, Chitinase, Cellulase, Protease, Amylase and Pectinase was recorded from selected strain VSMKU-2013. Phenazine antibiotic genes were indentified in VSMKU-2013 through DNA amplification by Polymerase chain reaction using specific universal primer of Phenazine.  Biosynthesis of Phenazine was visualized   through bioanalytical method of Thin Layer Chromatography (TLC) with Rf value 0.5 compare with that of authentic Phenazine.

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Name of the Staff: H. Harikrishnan

Name of the book: Bionanotechnology

Title of the chapter: Biosynthesis of Silver Nanoparticles from Fluorescent Pseudomonads against Phyto Fungal Pathogens. Name of the Publisher: Bloomsbury Publications

Year of Publication: 2016

Abstract:

In recent years nanotechnology has been emerging as a rapidly growing field with numerous applications in science and technology for the purpose of manufacturing new materials. This technology deals with the design, characterization and application of structures, devices and systems by controlling shape and size at nanometre scale level. It has been already found practical application in health and daily life, such as better drug delivery methods, chemical deposition for pollution clean up, medical, imaging as well as military purposes.

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Haseena, A., Nishad, V.M. and Balasundaran M. IOSR Journal of Environmental Science, Toxicology and Food Technology. 10: 49-59. A consortium of thermophilic microorganisms for aerobic composting. 2016

 

 

 Suma Arundev, Muralidharan, E.M., Sujanapal & Balasundaran, M. Annals of Forest Science 71:517-522. Identification of market adulterants in East Indian Sandal wood using DNA barcoding. 2014.

 

Sreekanth, P.M., Balasundaran, M. and Nazeem, P.A. Journal of Forestry Research 25 (4): 805-812. Genetic and morphological variation in natural teak (Tectona grandis) populations of the Western Ghats in Southern India. 2014.

 

Sreekanth, P.M, Balasundaran, M.,  Nazeem, P.A. and Suma, T.B. Conservation Genetics. 13:1409-1419. Genetic diversity of nine natural Tectona grandis L.f. populations of the Western Ghats in Southern India. 2012

 

Sreekanth, P.M, Balasundaran, M. Indian Journal of Genetics and Plant Breeding 72 (4): 1-5. AFLP diversity of teak clonal seed orchards. 2012.

 

Anupama C., Balasundaran, M. and Sujanapal, P. International Journal of Agriculture and Forestry 2: 150-156. Phylogenetic Relationships of Santalum album and its Adulterants as Inferred from Nuclear DNA Sequences. 2012.

 

Parvathy, M., Anupama, C. and Balasundaran, M. Molecular characterization of the differences between Santalum album L. and its adulterant, Osyris wightiana Wall. Ex. Wight using RAPD markers. Journal of Non-Timber Forest Products. 18 : 91-94.

 

Balasundaran, M., Jayson, E.A., Jayahari, K.M., Brinda, C.M. and Arathy, K.  REYONO journal of interdisciplinary studies. 1:141-150.  Non-invasive genotyping and genetic diversity of elephants: A study of 43 captive elephants. 2012.

 

Balakrishna, N., Balasundaran, M., Singh, R.K., Maikhuri, R.K., Shankar, S., Govindasami, V., Devayani Sen, Binisha, S. and Chandra, A. Soil Biol. Ecol. (Special Volume): 109-116. Assessment of abundance and diversity of legume nodulating bacteria (LNB) in Nilgiri and Nandadevi Biospheres of India across a gradient of land use intensity. 2009.

 

Dinesh Babu, K; Parameswaran, K; Rajeevan, P.K; Radha, T; Augustin, A; Balasundaran, M. Environment & Ecology 25(2): 250-253. Effect of enzyme α-amylase on the promotion of polyembryony in mango (Mangifera indica L.). 2007.

 

Sreekumar, V.B., Renuka, C., Suma, T.B. and Balasundaran, M. Botanical Studies 47: 443-452.Taxonomic reconsideration of Calamus rivalis Thw. ex  Trim. And C. metzianus Schlecht (Arecaceae) through morphometric and molecular analyses. 2006.

 

 Suma, T.B. and Balasundaran, M. Plant Cell Biotechnology and Molecular Biology 5:101-108. Biochemical and molecular studies during ontogeny of somatic embryos in sandal, Santalum album.  2004.

 

Mendham, D.S., Kumaraswamy, S., Balasundaran, M., Sankaran, K.V.,  Gerbeels, M., Grove, T.S.,  O’ Connell, A.M. and Rance, S.J. Biology and Fertility of Soils 39: 375-382. Legume cover cropping effects on early growth and soil nitrogen supply in eucalypt plantation in South-Western India. 2004.

 

Suma, T.B. and Balasundaran, M. Indian J. Genetics and Plant Breeding- 64: 167-168.Genetic diversity of eight Santalum album L. provenances of India based on RAPD analysis. 2004.

 

 Suma, T.B. and Balasundaran, M.    Indian J. Genetics and Plant Breeding 64(1): 13-16. Isozyme variation in seven Santalum  album L. populations of Kerala.  2004.

 

 Suma, T.B. and Balasundaran, M. Australian Journal of Botany 51:243-249.Isozyme variation in five provenances of Santalum album in India. 2003.

 

Thomas, S. and M. Balasundaran. Plant Cell Biotechnology and Molecular Biology. 2:145-148. Screening for phytoplasma in host plants of spike disease affected sandal. 2001.

 

Thomas, S. and M. Balasundaran. Plant Cell Biotechnology and Molecular Biology. 2:41-48. Detection of sandal spike phytoplasma by immunomicroscopy. 2001.

 

Thomas, S. and M. Balasundaran, Curr. Sci. 80:1489-1494. Purification of  sandal spike phytoplasma for the production of polyclonal antibody. 2001.

 

Thomas, S. and M. Balasundaran, Curr. Sci. 76: 1574-1576. Detection of sandal spike phytoplasma by polymerase chain reaction. 1999.

 

 

 

 

 

 

 

 

 

 

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